TNRK (normal rat kidney) epithelial cells stained with Acridine Orange. The dye fluoresces red by itself and the cargo vesicles that took up the dye are visible in red in the cytoplasm of the cells. The dye fluoresces green when bound to double-stranded nucleic acid and one can see the nuclei of the cells (ovals) containing the chromatin and the nucleoli inside the nuclei (brighter) fluorescing in green. Image: Prof. Falk and his 2020 Spring BioS368 students |
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