All of the routines in the book Method of Lines PDE Analysis in Biomedical Science and Engineering are written in R, an open source scientific computing system. Thus, it will be necessary to download R from the Internet, as explained in the following instructions (R is available for Windows, Mac and Linux). Instructions follow for installing and executing: (1) R, a general-purpose scientific computing system (2) Rstudio, a recommended editor for R (3) The R routines for Method of Lines PDE Analysis in Biomedical Science and Engineering, i.e, folders chap1 to chap10, For downloading R, Rstudio, and using the R routines: (1) Home page for R: http://www.r-project.org/ Then go to download link. (2) Direct link to R download link: http://cran.fhcrc.org/ In R, use "Packages" link to install the library for ordinary differential equations (ODEs): deSolve (3) To download Rstudio http://rstudio.org/ Select one of the following: RStudio 0.99.491 - Windows Vista/7/8/10 73.9 MB (dated 2015-12-30) RStudio 0.99.491 - Mac OS X 10.6+ (64-bit) 56.2 MB (dated 2015-12-30) In Rstudio, use "Packages" link to install the library for ordinary differential equations (ODEs): deSolve (4) To use the R files in folders chap1 to chap10, copy and paste the main programs, e.g., pde_1a_main.R in folder chap1, into R at the prompt and use "Enter". Note that it will first be necessary to edit the setwd (set working directory) in each main program to define the location (directory, folder) where the R routines reside. Alternatively, the main program can be copied and pasted into the R window (followed by "Enter") - that is, Rstudio is not used to execute the R routines, but this will circumvent the excellent graphics via "Plots" in Rstudio. Please direct any questions to wes1@lehigh.edu, (610) 758-4264.