We have previously shown the a-L-fucosidases of human semen include distinctive isoforms that exist (a) in the human seminal plasma, and (b) in the membranes of human sperm cells. Provocative circumstantial evidence on the distinctive fucosidases of the sperm cell and the seminal fluid suggested that this common enzyme may facilitate sperm transport and sperm-egg interactions. We recently showed that the Sperm Membrane Alpha-L-Fucosidase (SMALF) is enriched within sperm membrane system, and concentrated within the sperm segment. The a-L-fuosidase enzyme funtion is stabilized by its native association with the equatorial segment. Inhibitor studies gave strong evidence that hamster SMALF is not important for sperm-zona binding, but rather functions at the level of sperm-oocyte membrane-to-membrane interaction and/or post fusion events.
We have demonstrated the presence of seminal fucosidase in several species of fishes, which offer advantages for certain experimental approaches to the events of fertilization.
Our applied science interests include development of improved methods for contraeption. Most recently we have examined novel compounds with potential as spermicides and microbicides.
Khunsook, S., B. Bean, S.R. McGowan & J.A. Alhadeff, 2003. Purification and Characterization of Plasma Membrane-Associated Human Sperm alpha-L-Fucosidase. Biology of Reproduction 68:709-716.
Khunsook, S., J.A. Alhadeff, & B. Bean, 2002. Purification and Characterization of Human Seminal Plasma alpha-L-fucosidase. Molecular Human Reproduction, 8:221-227.
Khunsook, S., J.A. Alhadeff & B.S. Bean, 2001. Comparative characterization of the purified alpha-L-fucosidases that occur in the human sperm plasma membrane versus the seminal plasma. Molecular Biology of the Cell 12 Supplement 233a [abstract].
Khunsook, Sumpars, 2001. Purification and Characterization of Human Sperm Plasma Membrane-Associated and Human Seminal Fluid alpha-L-fucosidases. Lehigh University Doctoral Dissertation, 97pp. University Microfilms, 2001.
Alhadeff, J.A., Khunsook, S., Choowongkomon, K., Baney, T., Heredia, V., Tweedie, A., and Bean, B., 1999. Characterization of human semen alpha-L-fucosidases. Molecular Human Reproduction 5:809-815.
Bean, B., W. Li, S. Gibson, & J. Harris, 1999. Recombinant human ZPC induces the acrosome reaction in human sperm. J. Andrology 20suppl, 44. (Abstract)
Bean, B., S. Khunsook, K. Grimm, K. Choowongkomon, T. Baney, V. Heredia, A. Tweedie, & J.A. Alhadeff,1999. Characterization of alpha-L-fucosidases of human semen. J. Andrology 20suppl, 51. (Abstract)
Tang, S. & B. Bean, 1998. A panel of monoclonal antibodies against human sperm. Journal of Andrology 19:189-195.
Schneider, J.E., Goldman, M.D., Tang, S., Bean, B., Ji, H., and Friedman, M., 1998. Leptin Indirectly Affects Estrous Cycles by Increasing Metabolic Fuel Oxidation. Hormones and Behavior 33:217-228.
McGowan, S. & B. Bean, 1997. Initial characterization of a human sperm acrosomal antigen. Molecular Biology of the Cell 8: supplement p 106a.
Bean, B., 1994. The case for anti-sperm immunocontraception. Reproductive Health Matters 4:112-113.
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